Granulocyte-activating mediators (GRAM)

In the present study we investigated the capability of human epidermal cells to generate granulocyte-activating mediators (GRAM). It could be shown that human epidermal cells as well as an epidermoid carcinoma cell line (A431) produce an epidermal cell-derived granulocyte-activating mediator (EC-GRAM) which stimulates human granulocytes to release significant levels of toxic oxygen radicals as measured by a lucigenin-dependent chemiluminescence (CL). For further characterization of EC-GRAM the A431 cell line was used. Supernatants of A431 cells usually contained maximal EC-GRAM levels within 24 h of incubation. Factor production was enhanced by bacterial lipopolysaccharide (LPS), but not by silica particles and PHA. Moreover, freeze-thaw lysates of A431 cells and extracts of heat-separated human epidermis contained significant levels of EC-GRAM. Preincubation of granulocytes with EC-GRAM resulted in an enhanced response to subsequent stimulation with the chemotactic peptide f-met-phe. In contrast EC-GRAM did not affect the response to PMA or zymosan particles. However, EC-GRAM treated granulocytes were unresponsive to restimulation with EC-GRAM. Upon high performance liquid chromatography (HPLC) gel filtration EC-GRAM eluted within two major peaks exhibiting a molecular weight of 17 kD and 44 kD. According to its biochemical and biological properties EC-GRAM can be separated from other cytokines such as ETAF/-interleukin 1, interleukin 2, interferons, granulocyte colony-stimulating factor (G-CSF) and tumor necrosis factor (TNF). However, an antibody to human GM-CSF neutralized about 75% of the activity. These results indicate that EC-GRAM activity stimulating the generation of reactive oxygen species by granulocytes is probably due to GM-CSF

Introduction

Introduction by Robert A. Kap

Pathways to Prison: Life Histories of Former Clients of the Child Welfare and Juvenile Justice Systems

This study examines the relationship between child maltreatment and future offending from the viewpoint of former clients. Imprisoned adults describe their experiences in child welfare and juvenile justice system services. Specifically, those placed out of the home originally into the child welfare system have a different perspective on their path to prison than those placed into the juvenile justice system as delinquents. The study contributes to the literature by examining the relationship between the services children receive in the child welfare system as well as the juvenile justice system and their imprisonment as adults from a former service recipient\u27s point of view

History, Generations, and China Stories

In early May, a conference was held at Yale for retiring Chinese historian Jonathan Spence, with several China Beatniks in attendance. Here, Robert Kapp, one of Jonathan Spence’s first graduate students, reflects on the shifts in the stories we’ve told and heard about China during the time that Spence has been active in the field. The retirement of a distinguished scholar and doctoral mentor sometimes goes insufficiently remarked, but in the case of Jonathan Spence’s recent retirement from the Yale History faculty, something better happened. Happily, several of Spence’s Ph.D. students decided to throw their efforts into a conference and celebration in his honor, on the Yale Campus, in early May. The result was a most interesting and varied set of scholarly presentations, a warm and enthusiasticdinner event seasoned with warm reminiscences from generations of young and mid-career Chinese history scholars who received their early training from Spence, and a great many reunions of old friends with shared experiences of graduate life at Yale. Four attendees in particular – Robert Oxnam, Roger DesForges, Sherman Cochran, and I – represented the original tranche of doctoral candidates who finished their degrees under Jonathan’s benign and helpful guidance. We were far and away the oldest Spence “products” in attendance; all of us began our graduate school lives as students of Spence’s own academic mentors, Professors Arthur F. and Mary C. Wright. Jonathan essentially inherited us from Mary Wright, in particular, as both we and Yale lost an inspiring senior scholar and came to know a brilliant and promising one at the start of what would become a brilliant career in the China field. While most of the panels at the conference honoring Jonathan Spence consisted of research presentations – many of them on topics, and using tools of scholarly sleuthing – reminiscent of Jonathan’s own compelling works, the last session addressed “China Beyond the Academy,” in the form of a round table with five of Jonathan’s “products” who, over the years, either left academia altogether or who, while remaining active academics, engaged with broader audiences as a part of their China commitment

History, Generations, and China Stories

In early May, a conference was held at Yale for retiring Chinese historian Jonathan Spence, with several China Beatniks in attendance. Here, Robert Kapp, one of Jonathan Spence’s first graduate students, reflects on the shifts in the stories we’ve told and heard about China during the time that Spence has been active in the field. The retirement of a distinguished scholar and doctoral mentor sometimes goes insufficiently remarked, but in the case of Jonathan Spence’s recent retirement from the Yale History faculty, something better happened. Happily, several of Spence’s Ph.D. students decided to throw their efforts into a conference and celebration in his honor, on the Yale Campus, in early May. The result was a most interesting and varied set of scholarly presentations, a warm and enthusiasticdinner event seasoned with warm reminiscences from generations of young and mid-career Chinese history scholars who received their early training from Spence, and a great many reunions of old friends with shared experiences of graduate life at Yale. Four attendees in particular – Robert Oxnam, Roger DesForges, Sherman Cochran, and I – represented the original tranche of doctoral candidates who finished their degrees under Jonathan’s benign and helpful guidance. We were far and away the oldest Spence “products” in attendance; all of us began our graduate school lives as students of Spence’s own academic mentors, Professors Arthur F. and Mary C. Wright. Jonathan essentially inherited us from Mary Wright, in particular, as both we and Yale lost an inspiring senior scholar and came to know a brilliant and promising one at the start of what would become a brilliant career in the China field. While most of the panels at the conference honoring Jonathan Spence consisted of research presentations – many of them on topics, and using tools of scholarly sleuthing – reminiscent of Jonathan’s own compelling works, the last session addressed “China Beyond the Academy,” in the form of a round table with five of Jonathan’s “products” who, over the years, either left academia altogether or who, while remaining active academics, engaged with broader audiences as a part of their China commitment

Human Granulocyte-Macrophage Colony Stimulating Factor: An Effective Direct Activator of Human Polymorphonuclear Neutrophilic Granulocytes

Granulocyet-macrophage colony-stimulating factor (GM-CSF) was shown to modulate different granulocyte functions. In the present study we investigated the effect of purified and recombinant human GM-CSF, particularly on the oxidative metabolism of isolated human granulocytes. In addition, ultrastructural changes upon stimulation were evaluated. For detection of granulocyte activation the following assay systems were used: 1) lucigenin-dependent chemiluminescence (CL),2) superoxide-dismutase (SOD) inhibitable cytochrome C-reduction (superoxide),3) horseradish peroxidase-mediated oxidation of phenol red (hydrogen peroxide),4) release of myeloperoxidase, 5) ultrastructural detection of hydrogen peroxide-production, and 6) scanning and transmission electron microscopy (SEM and TEM, respectively). A significant CL response was seen upon stimulation with recombinant human GM-CSF at concentrations ranging from 1 to 103 U/ml. The CL response started within 5-10 min with a maximum at 60 – 90 min and lasted more than 3 h. Thereafter granulocytes were completely deactivated to restimulation with the same mediator and with Tumor Necrosis Factor, but respondend to other triggers of the oxidative burst, whereas the response to f-met-leu-phe was significantly increased, The CL signal was completely blocked by an antiserum to GM-CSF. Moreover, the response was significantly inhibited by SOD and D-mannitol, suggesting the involvement of distinct reactive oxygen species (ROS) in generating the CL response. Significant amounts of superoxide were detected within 180 min after stimulation with GM-CSF, whereas, release of hydrogen peroxide and peroxidase were only minimal as shown by functional and ultrastructural assays. Activation of granulocytes could be visualized by SEM and TEM. GM-CSF stimulated cells showed an increased adherence to the substratum developing polarized filopodia and an increased number of intercellular vesicles within 30 min after addition of the stimulus. The results clearly demonstrate that GM-CSF directly stimulates granulocytes and, particularly, their oxidative metabolism. Therefore, GM-CSF which is probably released by epidermal cells appears to be a candidate for neutrophil activation in the skin, and thereby may play a crucial role in inflammatory skin diseases

Measuring Consumer Satisfaction in Family Preservation Services: Identifying Instrument Domains

Measuring consumer satisfaction in the social services has become an important source of feedback for the improvement of service delivery. Consumer satisfaction has recently been incorporated into family preservation evaluation. This article reviews instruments used to measure consumer satisfaction in family preservation services and other related areas. Trends in current practice are examined and instrument dimensions are identified. Finally, some recommendations are made about the application of consumer satisfaction measurement in family preservation services